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Effect of SLC12A2 knockdown on inflammatory factor release and blood-retinal barrier in HG-treated rat retinal microvascular endothelial cells. (A) Levels of VEGF, IL-1β, IL-6 and TNF-α detected by ELISA. (B) Immunofluorescence staining showing the ZO-1 expression in each group. (C) Levels of <t>MMP2</t> and MMP9 detected by commercial kits. Data from three independent experiments are indicated as the mean ± SD (n=3). * P<0.05 and ** P<0.01. HG, high glucose; VEGF, vascular endothelial growth factor; IL-1β, interleukin-1Beta; IL-6, interleukin-6; TNF-α, tumor necrosis factor-alpha; ZO-1, zonula occludens-1; MMP, matrix metalloproteinase; n.s., not significant.
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Effect of SLC12A2 knockdown on inflammatory factor release and blood-retinal barrier in HG-treated rat retinal microvascular endothelial cells. (A) Levels of VEGF, IL-1β, IL-6 and TNF-α detected by ELISA. (B) Immunofluorescence staining showing the ZO-1 expression in each group. (C) Levels of MMP2 and MMP9 detected by commercial kits. Data from three independent experiments are indicated as the mean ± SD (n=3). * P<0.05 and ** P<0.01. HG, high glucose; VEGF, vascular endothelial growth factor; IL-1β, interleukin-1Beta; IL-6, interleukin-6; TNF-α, tumor necrosis factor-alpha; ZO-1, zonula occludens-1; MMP, matrix metalloproteinase; n.s., not significant.

Journal: International Journal of Molecular Medicine

Article Title: Bumetanide-blocked SLC12A2 exerts a protective effect in experimental diabetic retinopathy

doi: 10.3892/ijmm.2026.5774

Figure Lengend Snippet: Effect of SLC12A2 knockdown on inflammatory factor release and blood-retinal barrier in HG-treated rat retinal microvascular endothelial cells. (A) Levels of VEGF, IL-1β, IL-6 and TNF-α detected by ELISA. (B) Immunofluorescence staining showing the ZO-1 expression in each group. (C) Levels of MMP2 and MMP9 detected by commercial kits. Data from three independent experiments are indicated as the mean ± SD (n=3). * P<0.05 and ** P<0.01. HG, high glucose; VEGF, vascular endothelial growth factor; IL-1β, interleukin-1Beta; IL-6, interleukin-6; TNF-α, tumor necrosis factor-alpha; ZO-1, zonula occludens-1; MMP, matrix metalloproteinase; n.s., not significant.

Article Snippet: In the present study, kits for MMP2 (cat. no. E-EL-R0618) and MMP9 (cat. no. E-EL-R3021; both from Elabscience Biotechnology, Inc.) were employed to detect the activity of these two factors in cell supernatants.

Techniques: Knockdown, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing